Thermo Fisher Scientific Invitrogen Taq DNA Polymerase, recombinant 500 Reactions
Taq DNA Polymerase, recombinant, is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. This recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli and consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5' to 3' DNA polymerase activity and a 5' to 3' exonuclease activity.
Features
- A choice of recombinant or native enzyme
- Amplification of PCR products up to 5 kb in size
- Enzyme is licensed and qualified for PCR
Applications
- Amplification of DNA from complex genomic, viral, and plasmid templates
- RT-PCR
- Sequencing ssDNA
- Cycle sequencing
Notes
- One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 minutes at 74°C.
- For superior PCR performance, DreamTaq DNA Polymerase is recommended.
For Research Use Only. Not for use in diagnostic procedures.
| Format | Stand-alone enzyme |
|---|---|
| GC-Rich PCR Performance | Low |
| PCR Method | qPCR, Standard PCR |
| Polymerase | Taq DNA Polymerase |
| Reaction Speed | Standard |
| Exonuclease Activity | 5' - 3' |
| Product Type | DNA Polymerase |
| Quantity | 500 Units |
| Shipping Condition | Dry Ice |
| For Use With (Application) | Standard PCR |
| Concentration | 5 U/μL |
| Fidelity (vs. Taq) | 1X |
| Hot Start | No |
| No. of Reactions | 500 Reactions |
| Overhang | 3'-A |
| Reaction Format | Separate Components |
| Size (Final Product) | 5 kb or less |
| Starting Material | DNA |
| Unit Size | 500 units |