Thermo Fisher Scientific DMEM/F-12, HEPES
Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco™ DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM / F-12 is modified as follows:
| With |
| • L-glutamine |
| • HEPES |
| • Phenol Red |
The complete formulation is available.Gibco™ DMEM / F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components.
DMEM / F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM / F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO2 environment to maintain physiological pH.
| Classification | Animal Origin-free |
|---|---|
| Concentration | 1 X |
| Culture Type | Mammalian Cell Culture |
| Form | Liquid |
| Product Type | DMEM (Dulbecco's Modified Eagle Medium)/F-12 |
| Shelf Life | 12 Months |
| Sterility | Sterile-filtered |
| With Additives | Glutamine, HEPES, Phenol Red, High Glucose, Sodium Pyruvate |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Quantity | 500 mL |
| Unit Size | 500 mL |