Thermo Fisher Scientific Opti-MEM I Reduced Serum Medium
Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology. Opti-MEM™ I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM™ I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM™ I modifications for a range of cell culture applications.
This Opti-MEM™ I is modified as follows:
| With |
| • L-glutamine |
| • Phenol Red |
The complete formulation is confidential. For more information, please contact Technical Services.
Using Opti-MEM™ I Medium
Opti-MEM™ I Reduced-Serum Medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM™ I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
| Cell Line | Sp2, AE-1, CHO, BHK-21, and HEK |
|---|---|
| Cell Type | Primary Fibroblasts |
| Classification | Human Origin |
| Concentration | 1 X |
| Form | Liquid |
| Product Type | Opti-MEM I |
| Shelf Life | 18 Months From Date of Manufacture |
| Sterility | Sterile-filtered |
| With Additives | Glutamine, Phenol Red |
| Green Features | Sustainable packaging |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Product Line | Gibco™, Opti-MEM™ |
| Quantity | 500 mL |
| Shipping Condition | Room Temperature |
| Unit Size | 500 mL |