Thermo Fisher Scientific MultiScribe Reverse Transcriptase
MultiScribe Reverse Transcriptase is a recombinant moloney murine leukemia virus (rMoMuLV) reverse transcriptase optimized for TaqMan-based assays. MultiScribe Reverse Transcriptase is similar to MuLV reverse transcriptase but differs in its recommended usage. This recombinant RNA–dependent, DNA polymerase uses single-stranded RNA as a template in the presence of a primer to synthesize a complementary DNA (cDNA) strand.
Features of MultiScribe Reverse Transcriptase include:
• Compatible with one-step and two-step RT-PCR reactions, which provides flexibility in assay setup
• Individual component design provides versatility in assay setup
• 5,000 U is supplied in 100 μL vials (50 U/μL)
• Reaction temperature up to 52°C
Applications
• PCR
• RT-PCR
• Reverse Transcription
| Format | Stand-alone Enzyme |
|---|---|
| Technique | Reverse Transcription |
| Optimal Reaction Temperature | 37°C |
| Quantity | 100 μL |
| Reverse Transcriptase | MultiScribe |
| Ribonuclease H Activity | Reduced |
| Shipping Condition | Dry Ice |
| For Use With (Application) | RT-PCR |
| Concentration | 50 U/μL |
| Final Product Type | First-Strand cDNA |
| Reaction Format | Separate components |
| Reagent Type | Reverse Transcription |
| Size (Final Product) | Up to 7 kb |
| Starting Material | RNA |
| Unit Size | 100 µL |