PrepFiler LySep Columns are intended for use with the PrepFiler Forensic DNA Extraction kits and allow users to easily perform lysis and substrate removal in a single tube assembly. There is no transfer of the substrate to another tube for removal, thus minimizing hands-on time and helping to reduce the risk of cross contamination and sample transposition events. The only analyst interaction and handling of the substrate occurs during the addition of the substrate to the PrepFiler LySep Column for lysis.

The innovative PrepFiler LySep Column dramatically streamlines the off-line lysis portion of the extraction method. PrepFiler Lysis Buffer or PrepFiler BTA Lysis buffer is added directly to the PrepFiler LySep Column along with the sample for incubation. Next, the PrepFiler LySep Column is briefly centrifuged, causing the lysate to flow through the burstable membrane while the substrate remains behind in the column. The sample tube is then ready to be placed on the AutoMate Express instrument. This eliminates the manual lysate and substrate transfer steps, helping save time and minimizing cross contamination and sample transposition events.