Thermo Fisher Scientific Ion Torrent Ion Library Equalizer Kit 96 Reactions
The Ion Library Equalizer™ Kit is a simple and seamless bead-based solution replacing the need for library quantification and library dilutions for library normalization as required for any next generation sequencing workflow. The Ion Library Equalizer™ Kit helps reduce labor, reagent costs, and further simplifies the Ion semiconductor sequencing workflow for high sample-throughput or barcoded projects, as well as single or low sample number projects.
The kit workflow consists of three simple steps:
1) Library amplification with Equalizer™ Primers
2) Library capture onto Equalizer™ Beads
3) Heat elution of the normalized library using a specially formulated Equalizer™ Elution Buffer
Simple Workflow
• Seamlessly integrates into your existing library preparation workflow
• Reduced pipetting compared to the Agilent 2100 Bioanalyzer™-based workflow
• Faster workflow in high-throughput applications-no need for DNA quantification, calculations, and dilution of DNA libraries
• Minimizes library-to-library variability
Cost Effective
• No additional capital equipment required
• Less expensive than Agilent 2100 Bioanalyzer™-based workflow (up to 10 samples)
• Reduced AMPure™ XP reagent usage compared to Agilent 2100 Bioanalyzer™-based workflow
• Long shelf life: up to 18 months
• Convenient 96-reaction kit helps reduce ordering frequency
Flexible
• Compatible with all Ion DNA library kits including Ion AmpliSeq™ DNA panels, Ion fragment library kits, and Ion Xpress™ Barcode adaptors
• Use with a single sample or in a 96-well plate format
• Compatible with 3rd party automated liquid handling systems for integrated library preparation and normalization
Includes:
The Ion Library Equalizer Kit includes the necessary reagents to complete 96 normalization reactions.
Required for Workflow:
Ion AmpliSeq™ Library Kit 2.0
Ion AmpliSeq™ Ready-to-Use Panel or Custom Panel designed via the Ion AmpliSeq™ Designer (www.ampliseq.com)
--OR--
Ion Plus Fragment Library Kit
--OR--
Ion Xpress™ Plus Fragment Library Kit
Optional:
Ion Xpress™ Barcode Adapters Kits
The kit workflow consists of three simple steps:
1) Library amplification with Equalizer™ Primers
2) Library capture onto Equalizer™ Beads
3) Heat elution of the normalized library using a specially formulated Equalizer™ Elution Buffer
Simple Workflow
• Seamlessly integrates into your existing library preparation workflow
• Reduced pipetting compared to the Agilent 2100 Bioanalyzer™-based workflow
• Faster workflow in high-throughput applications-no need for DNA quantification, calculations, and dilution of DNA libraries
• Minimizes library-to-library variability
Cost Effective
• No additional capital equipment required
• Less expensive than Agilent 2100 Bioanalyzer™-based workflow (up to 10 samples)
• Reduced AMPure™ XP reagent usage compared to Agilent 2100 Bioanalyzer™-based workflow
• Long shelf life: up to 18 months
• Convenient 96-reaction kit helps reduce ordering frequency
Flexible
• Compatible with all Ion DNA library kits including Ion AmpliSeq™ DNA panels, Ion fragment library kits, and Ion Xpress™ Barcode adaptors
• Use with a single sample or in a 96-well plate format
• Compatible with 3rd party automated liquid handling systems for integrated library preparation and normalization
Includes:
The Ion Library Equalizer Kit includes the necessary reagents to complete 96 normalization reactions.
Required for Workflow:
Ion AmpliSeq™ Library Kit 2.0
Ion AmpliSeq™ Ready-to-Use Panel or Custom Panel designed via the Ion AmpliSeq™ Designer (www.ampliseq.com)
--OR--
Ion Plus Fragment Library Kit
--OR--
Ion Xpress™ Plus Fragment Library Kit
Optional:
Ion Xpress™ Barcode Adapters Kits
For Research Use Only. Not for use in diagnostic procedures.
| Product Line | Equalizer™ |
|---|---|
| Starting Material | DNA |
| Format | 96-well Plate |
| For Use With (Application) | Amplicon Sequencing |
| For Use With (Equipment) | Ion PGM™ System, Ion Proton™ System, Ion Personal Genome Machine (PGM™) System |
| Libraries | Fragment Library, Targeted Sequencing Library, Normalized Library |
| Multiplex Capability | No Limit on Multiplexing |
| Product Type | Library Equalizer Kit |
| Quantity | 96 reactions |
| Sample Type | Any Sample Type |
| Sample Volume | Fragment or Targeted AmpliSeq Libraries |
| Sequencing Type | Epigenetic Sequencing, Genome & DNA Sequencing |
| Species | All Species |
| Throughput | Up to 10 Samples |
| Workflow Step | Library Barcoding, Library Generation, Template Preparation |
| Unit Size | 96 reactions |