Thermo Fisher Scientific Invitrogen RNAqueous Total RNA Isolation Kit
The RNAqueous™ Kit is for phenol-free total RNA isolation from 1–75 mg of tissue or from 102–107 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The RNAqueous™ Kit provides:
• Convenient syringe-based filtration
• Compatibility with vacuum manifolds when processing multiple samples
How it works
The RNAqueous™ Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.
• Convenient syringe-based filtration
• Compatibility with vacuum manifolds when processing multiple samples
How it works
The RNAqueous™ Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.
For Research Use Only. Not for use in diagnostic procedures.
| Isolation Technology | Spin Column (Glass Fiber Filter) |
|---|---|
| Elution Volume | 50–120 μL |
| Sample Type | Bacteria, Cells, Plant, Tissue, Yeast, Viruses, Enzymatic Reactions |
| Final Product Type | Total RNA |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| For Use With (Application) | RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling |
| Quantity | 50 Preps |
| Shipping Condition | Room Temperature |
| Starting Material Amount | Bacteria: ≤109 cellsCells: ≤107Tissue: ≤75 mgPlant: ≤75 mgYeast: ≤108 cellsVirus: variesEnzymatic Reactions: varies |
| Unit Size | 50 preps |