Thermo Fisher Scientific GeneJET RNA Purification Kit
Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.
The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.
RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.
Highlights
• Universal—can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and bacteria
• Efficient—high yields of total RNA.
• Fast—15 minutes after lysis step
• Pure—an A260/280 ratio > 1.9
• Convenient—spin columns are capped and assembled with collection tubes
Applications
Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assay
Includes
Proteinase K Lysis Buffer, Wash Buffer 1 (concentrated), Wash Buffer 2 (concentrated), Nuclease-free Water, GeneJET RNA Purification Columns (pre-assembled with Collection Tubes), Collection Tubes (2 mL), Collection Tubes (1.5 mL), Detailed Protocol
| Isolation Technology | Silica Spin Column |
|---|---|
| Purification Time | 15 min. (After sample homogenization) |
| Elution Volume | 50–100 μL |
| Sample Type | Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions |
| Final Product Type | Total RNA |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| For Use With (Application) | RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling |
| Quantity | 50 Preps |
| Scale | Mini |
| Shipping Condition | Room Temperature |
| Starting Material Amount | Bacteria: ≤109 cellsBlood: 0.5 mLCells: ≤107Tissue and Insects: 30 mgYeast: ≤4 x 108 cellsEnzymatic reactions: ≤100 μL |
| Yield | Up to 160 μg |
| Unit Size | 50 preps |